Our Members:
Denisa D. Wagner, PhD - Principal Investigator
Dr. Wagner received her Diploma of Biochemistry from the Universite de Geneve, Switzerland and PhD in Biology from the Massachusetts Institute of Technology. She served on the faculty at the University of Rochester, NY and Tufts University School of Medicine before coming to Harvard Medical School in 1994. Dr. Wagner is currently the Edwin Cohn Professor of Pediatrics in the Program for Cellular and Molecular Medicine and the Division of Hematology/Oncology at Boston Children's Hospital.
E-mail: denisa.wagner@childrens.harvard.edu
Dr. Wagner received her Diploma of Biochemistry from the Universite de Geneve, Switzerland and PhD in Biology from the Massachusetts Institute of Technology. She served on the faculty at the University of Rochester, NY and Tufts University School of Medicine before coming to Harvard Medical School in 1994. Dr. Wagner is currently the Edwin Cohn Professor of Pediatrics in the Program for Cellular and Molecular Medicine and the Division of Hematology/Oncology at Boston Children's Hospital.
E-mail: denisa.wagner@childrens.harvard.edu
Siu Ling (Christine) Wong, PhD - Instructor
Started in Wagner Lab: 08/2011
Christine came to the Wagner Lab from Hong Kong as a postdoctoral fellow to explore the impact of blood cells on inflammation. She is particularly interested in how diabetes affects the formation of neutrophil extracellular traps (NETs) and how NETs may contribute to diabetic complications.
Previous research:
The role of cyclooxygenase-2 in endothelial dysfunction and vascular inflammation
Previous placements:
The Chinese University of Hong Kong, Hong Kong - B.S. in Biology (Human Biology)
The Chinese University of Hong Kong, Hong Kong - PhD in Physiology
Under the supervision of Dr. Yu Huang
E-mail: siuling.wong@childrens.harvard.edu
Started in Wagner Lab: 08/2011
Christine came to the Wagner Lab from Hong Kong as a postdoctoral fellow to explore the impact of blood cells on inflammation. She is particularly interested in how diabetes affects the formation of neutrophil extracellular traps (NETs) and how NETs may contribute to diabetic complications.
Previous research:
The role of cyclooxygenase-2 in endothelial dysfunction and vascular inflammation
Previous placements:
The Chinese University of Hong Kong, Hong Kong - B.S. in Biology (Human Biology)
The Chinese University of Hong Kong, Hong Kong - PhD in Physiology
Under the supervision of Dr. Yu Huang
E-mail: siuling.wong@childrens.harvard.edu
Deya Cherpokova, PhD - Post Doctoral Fellow
Started in Wagner Lab: 06/2016
Deya came to us from Bulgaria as a DFG fellow to study the impact of NETs on vascular fibrosis, systemic inflammation and secondary injury after venous thrombosis.
Previous research:
Deya studied the modulators of platelet (hem)ITAM signaling and platelet production
Previous placements:
University of Wuerzburg, Wuerzburg, Germany - B.S. and Master's in Biomedicine
University of Wuerzburg, Wuerzburg, Germany - PhD
Laboratory of Professor Bernhard Nieswandt
E-mail: deya.cherpokova@childrens.harvard.edu
Started in Wagner Lab: 06/2016
Deya came to us from Bulgaria as a DFG fellow to study the impact of NETs on vascular fibrosis, systemic inflammation and secondary injury after venous thrombosis.
Previous research:
Deya studied the modulators of platelet (hem)ITAM signaling and platelet production
Previous placements:
University of Wuerzburg, Wuerzburg, Germany - B.S. and Master's in Biomedicine
University of Wuerzburg, Wuerzburg, Germany - PhD
Laboratory of Professor Bernhard Nieswandt
E-mail: deya.cherpokova@childrens.harvard.edu
Daniella de Moraes Mizurini, PhD - Post Doctoral Fellow
Started in Wagner Lab: 03/2016
Daniella came to us from Brazil to study the role of neutrophil extracellular traps (NETs) and peptidylarginine deiminase 4 (PAD4) in stroke progression.
Previous research:
Daniella studied the characterization of molecular mechanisms involved in the antithrombotic activity of salivary protein from hematophagous sources.
Previous placements:
Federal University of Rio de Janeiro, Rio de Janeiro, Brazil - B.S. in Human Nutrition
Federal University of Rio de Janeiro, Rio de Janeiro, Brazil - PhD in Biochemistry
Laboratory of Thrombosis and Cancer
E-mail: daniella.demoraesmizurini@childrens.harvard.edu
Started in Wagner Lab: 03/2016
Daniella came to us from Brazil to study the role of neutrophil extracellular traps (NETs) and peptidylarginine deiminase 4 (PAD4) in stroke progression.
Previous research:
Daniella studied the characterization of molecular mechanisms involved in the antithrombotic activity of salivary protein from hematophagous sources.
Previous placements:
Federal University of Rio de Janeiro, Rio de Janeiro, Brazil - B.S. in Human Nutrition
Federal University of Rio de Janeiro, Rio de Janeiro, Brazil - PhD in Biochemistry
Laboratory of Thrombosis and Cancer
E-mail: daniella.demoraesmizurini@childrens.harvard.edu
Nicoletta Sorvillo, PhD - Post Doctoral Fellow
Started in Wagner Lab: 04/2015
Nicoletta came to the Wagner Lab from the Netherlands and Italy, as a postdoctoral fellow, in order to study the role of neutrophil extracellular traps (NETs) in the initiation and progression of thrombosis and inflammation. In particular, she is interested in the function of PAD4, the enzyme crucial for NET formation, outside the cell and to determine its effect on the extracellular environment.
Previous research:
Nicoletta studied potential triggers for development of auto-antibodies in acquired thrombotic thrombocytopenic purpura (TTP).
She determined the mechanism of endocytosis of ADAMTS13 by human dendritic cells and identified for the first time the repertoire of naturally MHC class II presented ADAMTS13-derived peptides.
Nicoletta also analyzed the immune recognition and processing of plasma proteins, such as von Willebrand factor and Factor VIII, for the design of improved therapeutics for coagulation disorders such as Hemophilia A.
Previous placements:
University of Naples Federico II, Naples, Italy – Masters of Medical Biotechnology
University of Amsterdam, Amsterdam, The Netherlands – PhD in Hematology and Immunology
Under the supervision of Professor Jan Voorberg at the Department of Plasma Proteins at Sanquin Research
Sanquin Research, Amsterdam, The Netherlands – Postdoctoral fellow
Under the supervision of Professor Koen Mertens and Dr. Sander Meijer with Novo Nordisk’s collaboration
E-mail: nicoletta.sorvillo@childrens.harvard.edu
Started in Wagner Lab: 04/2015
Nicoletta came to the Wagner Lab from the Netherlands and Italy, as a postdoctoral fellow, in order to study the role of neutrophil extracellular traps (NETs) in the initiation and progression of thrombosis and inflammation. In particular, she is interested in the function of PAD4, the enzyme crucial for NET formation, outside the cell and to determine its effect on the extracellular environment.
Previous research:
Nicoletta studied potential triggers for development of auto-antibodies in acquired thrombotic thrombocytopenic purpura (TTP).
She determined the mechanism of endocytosis of ADAMTS13 by human dendritic cells and identified for the first time the repertoire of naturally MHC class II presented ADAMTS13-derived peptides.
Nicoletta also analyzed the immune recognition and processing of plasma proteins, such as von Willebrand factor and Factor VIII, for the design of improved therapeutics for coagulation disorders such as Hemophilia A.
Previous placements:
University of Naples Federico II, Naples, Italy – Masters of Medical Biotechnology
University of Amsterdam, Amsterdam, The Netherlands – PhD in Hematology and Immunology
Under the supervision of Professor Jan Voorberg at the Department of Plasma Proteins at Sanquin Research
Sanquin Research, Amsterdam, The Netherlands – Postdoctoral fellow
Under the supervision of Professor Koen Mertens and Dr. Sander Meijer with Novo Nordisk’s collaboration
E-mail: nicoletta.sorvillo@childrens.harvard.edu
Patrick Münzer, PhD - Post Doctoral Fellow
Started in Wagner lab: 10/2017
Previous research:
Patrick's previous work focused on the molecular basis of hemostasis and pathological thrombosis with subsequent thrombo-occlusive events leading to myocardial infarction and ischemic stroke. He investigated the role of the Phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) pathway and associated kinases in platelet signaling and activation.
Previous placements:
The University of Freiburg, Germany- BS in Biology
The University of Tübingen, Germany- PhD in Physiology
Under the supervision of Prof. Florian Lang, MD
The University Hospital of Tübingen, Germany- Postdoctoral Research Fellow in Thrombosis/Hemostasis
Under the supervision of Prof. Meinrad Gawaz, MD
E-mail: patrick.muenzer@childrens.harvard.edu
Started in Wagner lab: 10/2017
Previous research:
Patrick's previous work focused on the molecular basis of hemostasis and pathological thrombosis with subsequent thrombo-occlusive events leading to myocardial infarction and ischemic stroke. He investigated the role of the Phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) pathway and associated kinases in platelet signaling and activation.
Previous placements:
The University of Freiburg, Germany- BS in Biology
The University of Tübingen, Germany- PhD in Physiology
Under the supervision of Prof. Florian Lang, MD
The University Hospital of Tübingen, Germany- Postdoctoral Research Fellow in Thrombosis/Hemostasis
Under the supervision of Prof. Meinrad Gawaz, MD
E-mail: patrick.muenzer@childrens.harvard.edu
Shoichi Fukui, MD, PhD - Post Doctoral Fellow
Started in Wagner lab: 06/2018
Shoichi came to the Wagner lab from Japan in order to study the relationship between neutrophil extracellular traps (NETs) and rheumatoid arthritis.
Previous research:
Shoichi studied subsets of monocytes and osteoclasts in rheumatoid arthritis using human peripheral blood samples. He also researched for vasculitis and complement pathways clinically.
Previous placements:
Nagasaki University, Nagasaki, Japan - MD
Nagasaki University, Nagasaki, Japan - PhD in Medicine
Immunology and Rheumatology, Professor Atsushi Kawakami
Post-doctoral career:
Assistant professor, Department of Community Medicine, Nagasaki University, Nagasaki, Japan
E-mail: shoichi.fukui@childrens.harvard.edu
Started in Wagner lab: 06/2018
Shoichi came to the Wagner lab from Japan in order to study the relationship between neutrophil extracellular traps (NETs) and rheumatoid arthritis.
Previous research:
Shoichi studied subsets of monocytes and osteoclasts in rheumatoid arthritis using human peripheral blood samples. He also researched for vasculitis and complement pathways clinically.
Previous placements:
Nagasaki University, Nagasaki, Japan - MD
Nagasaki University, Nagasaki, Japan - PhD in Medicine
Immunology and Rheumatology, Professor Atsushi Kawakami
Post-doctoral career:
Assistant professor, Department of Community Medicine, Nagasaki University, Nagasaki, Japan
E-mail: shoichi.fukui@childrens.harvard.edu
Long H. Chu, BS - Lab Manager
Started in Wagner Lab: 07/2016
Previous research:
This is Long's first posting in a research position in a lab.
Before becoming the lab manager, Long was a research assistant at Wagner Lab.
Previous placements:
UMass Lowell, Lowell, MA, USA - B.S. in Molecular Biology
Eton Bioscience Inc - Specializing in DNA Sequencing
E-mail: long.chu@childrens.harvard.edu
Started in Wagner Lab: 07/2016
Previous research:
This is Long's first posting in a research position in a lab.
Before becoming the lab manager, Long was a research assistant at Wagner Lab.
Previous placements:
UMass Lowell, Lowell, MA, USA - B.S. in Molecular Biology
Eton Bioscience Inc - Specializing in DNA Sequencing
E-mail: long.chu@childrens.harvard.edu
Caleb R. Staudinger, BS - Research Assistant
Started in Wagner Lab: 12/2015
Previous research:
This is Caleb's first posting in a research position in a lab.
Previous placements:
Carnegie Mellon University, Pittsburgh, PA, USA - B.S. in Biological Sciences
Taconic Biosciences - Specializing in laboratory animal maintenance and production
E-mail: caleb.staudinger@childrens.harvard.edu
Started in Wagner Lab: 12/2015
Previous research:
This is Caleb's first posting in a research position in a lab.
Previous placements:
Carnegie Mellon University, Pittsburgh, PA, USA - B.S. in Biological Sciences
Taconic Biosciences - Specializing in laboratory animal maintenance and production
E-mail: caleb.staudinger@childrens.harvard.edu